Micropipettes

For TREC Microdrives / for other Applications

Thomas microinjection pipettes are available for use with Thomas Microdrive systems (e.g. Mini Matrix, Eckhorn Matrix) or for other microdrives or manual electrode manipulators.

Beside microelectrodes the micropipettes can be used in our multielectrode manipulator systems (“System Eckhorn” or “Mini Matrix”), for drug injection through the pipette and simultaneous recording of the neural drug response with the microelectrodes.

The micropipette fiber has an outer diameter of 100-120µm and is made of quartz-glass or stainless steel. 

Key features:

  • Outer diameter of pipette fiber is 100-120µm
  • Very flexible quartz-glass fiber can be strengthened with metal tube or glass pipette to be mounted to a manual microdrive system
  • Available for use with any standard manual manipulator system or with Thomas RECORDING multielectrode microdrives (“System Eckhorn” or “Mini Matrix”)
  • Software controlled microinjection pump system available

Thomas microinjection technique uses either a metal needle or a quartz glass micropipette together with a microinjection pump to inject small liquid volumes. Typically a stereotaxic setup may be required. The Thomas microinjection pipettes are available for Thomas Microdrive systems or for other motorized or manual microdrives.

 

 

Pipettes for Thomas Microdrives

Microinjection pipettes for the Thomas microdrives are equipped with the patented Thomas rubber tube drive that allows to load the pipette to a Thomas Mini Matrix or Eckhorn Matrix drive (see figure 1 and 2). Microelectrodes and pipette are moved by the rubber tube of the microdrive to place them with micrometer precision in the animal´s brain or in a brain slice. The microinjections for the Thomas microdrives are available with stainless steel of quartz glass pipette material. We are stringent with quality parameters, only microinjection pipettes that pass the strict quality standards are released as final product.

 

 

Pipettes for other Microdrives

Microinjection pipettes for other than Thomas microdrives are also made of quartz glass or stainless steel (see figure 3-6). These microinjection pipettes (OD=100-120µm) are strengthened with an additional glass pipette (OD= 0.5, 1.0, 1.5 or 2.0mm, see figure 3 and 4) or a stainless steel tube (OD=305µm, see figure 5 and 6) so that you can clamp it to an electrode holder clamp of an electrode manipulator.

 

 

Figure 1: Components of the microinjection pipette for use with Thomas microdrives

 

 

Figure 2: Dimensions of microinjection pipette for use with Thomas microdrives

 

 

Figure 3: Components of the microinjection pipette for use with other microdrives, strengthened with glass pipette

 

 

Figure 4: Dimensions of microinjection pipette for use with other microdrives, strengthened with glass pipette

 

 

Figure 5: Components of the microinjection pipette for use with other microdrives, strengthened with metal tube

 

 

Figure 6: Dimensions of microinjection pipette for use with other microdrives, strengthened with metal tube

 

 

Publications

[1] Vera K. Veith, Cliodhna Quigley, Stefan Treue, A Pressure Injection System for Investigating the Neuropharmacology of Information Processing in Awake Behaving Macaque Monkey Cortex. Jove, 3/14/2016, Issue 109; doi: 10.3791/53724

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