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Artificial cerebrospinal fluid (ACSF)

Neuroscience Products > Accessories > Amplifiers > Chronic Recording Devices > Data Acquisition Systems > Eye Tracking Systems > Microdrives > Microelectrodes > Microinjection Systems> Optogenetic Products > Primate Training Systems > Screws > Software > Stereotaxic Instruments > Cell & Tissue Media > Rodent Products Medical Products Electrochemical Products

Artificial cerebrospinal fluid (ACSF)

Artificial cerebrospinal fluid (ACSF) is a biological buffer that enables a vital environment for neuronal tissue by maintaining the homeostasis, osmolarity, and pH at physiological levels. ACSF is commonly used for many lab experiments including in vitro and in vivo applications.

Product Information:

Ready to Use ACSF solution or 20x concentrate available

Carbogen (95% oxygen and 5% carbon dioxide) supply is necessary for proper Bicabonate pH buffering

autoclaved

 

Instructions for use:

Ready to use:

For 1000 ml ACSF Solution:

1. Oxygenate 500 ml Base A for 10 min

2. Add slowly 500 ml Base B

3. Oxygenate NH-BSRS Solution during use

20x concentrate:

For 1000 ml ACSF Solution:

1. Pour 50 ml Base A (20x) into a flask and add 900 ml deionized water

2. Add slowly 50 ml Base B (20x)

3. Oxygenate ACSF Solution during use

 

pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)

Use immediately once opened. Store at 4 – 8 °C

IONAL COMPOSITION OF ACSF SOLUTION

NaCl125 mM
KCl3 mM
CaCl22,5 mM
MgSO41,3 mM
NaH2PO41,25 mM
NaHCO326 mM
Glucose13 mM

Questions? Please don’t hesitate contacting us!

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