Sodium-free High Sucrose Brain Slice Cutting Solution (SHS-BSCS)

Sodium-free High Sucrose Brain Slice Cutting Solution (HS-BSCS) is used for cutting acute brain slices from rats and mice.

 

Product Information:

Ready to Use SHS-BSCS solution

autoclaved

 

Instructions for use:

Ready to use:

For 1000 ml SHS-BSCS Solution:

1. Oxygenate 500 ml Base A for 10 min

2. Add slowly 500 ml Base B

3. Oxygenate SHS-BSCS Solution during use

 

pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)

Use immediately once opened. Store at 4 - 8 °C

Ional composition of HS-BSCS Solution

Characteristic Value
Sucrose 234 mM
NaHCO3 26 mM
Glucose 11 mM
MgSO4 10 mM
NaH2PO4 1,25 mM
KCl 2,5 mM
CaCl2 0,5 mM
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Publications

[1] Aghajanian and Rasmussen, Synapse Vol. 3 (4), 331-338, 1989.


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