NMDG-HEPES Brain Slice Recovery Solution (NH-BSRS)

NMDG-HEPES Brain Slice Recovery Solution (1000 ml) (NH-BSRS) is used for recovery of acute brain slices from rats and mice after the cutting procedure.

 

Product Information:

Ready to Use NH-BSRS solution

autoclaved

 

Instructions for use:

Ready to use:

For 1000 ml NH-BSRS Solution:

1. Oxygenate 500 ml Base A for 10 min

2. Add slowly 500 ml Base B

3. Oxygenate NH-BSRS Solution during use

 

pH 7.4 when oxygenated with 95% O2, 5% CO2 (Carbogene)

Use immediately once opened. Store at 4 - 8 °C

Ional composition of NMDG-HEPES Brain Slice Recovery Solution

Characteristic Value
N-Methyl-D-Glucamine 93 mM
NaHCO3 30 mM
Glucose 25 mM
Na L-Ascorbate 5 mM
MgSO4 10 mM
NaH2PO4 1,25 mM
KCl 2,5 mM
CaCl2 0,5 mM
HEPES 20 mM
Thiourea 2 mM
Na Pyruvate 3 mM

 

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