Normal Frog Ringer (NFR)

Normal Frog Ringer (NFR) is used for lab experiments with Xenopus laevis oocytes to guarantee a physiological environment with respect to homeostasis, pH and osmolarity.

 

Product Information:

Ready to Use NFR solution or 10x concentrate available

autoclaved

 

Instructions for use:

Ready to use:

Direct use

10x concentrate:

For 1000 ml NFR Solution:

1. Pour 100 ml NFR (10x) into a flask

2. Add deionized water to a final volume of 1000 ml

 

Adjust to pH 7,4

Use immediately once opened. Store at 4 - 8 °C

Ional composition of NFR

Characteristic Value
NaCl 90 mM
KCl 2 mM
CaCl2 2 mM
MgCl2 1 mM
HEPES 5 mM

 

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